The retention volume of a solute is 76.1mL for a column withVm=16.6mL andVs=12.7mL . Calculate the retention factor and the partition coefficient for this solute.

Short Answer

Expert verified

a) Retention factor =k =3.59

b) Partition coefficient=k = 4.69

Step by step solution

01

Given Information:

Vr=76.2mL,Vm=16.6mL,Vs=12.7mL

02

To find the Retention factor:

Retention factor as

k=Vr-VmVm

Substitute the given data values are

k=76.2mL-16.6mL16.6mLk=3.59

03

To find the Partition coefficient;

K=k×VmVs

Substitute the given data values are

K=3.59×16.6mL12.7mLK=4.69

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23-41. Describe how nonlinear partition isotherms lead to non-Gaussian band shapes. Draw the chromatographic peak shapes produced by an overloaded gas chromatography column and an overloaded liquid chromatography column.

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