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Chapter 23: Q26P (page 630)

The retention volume of a solute is 76.1mL for a column withVm=16.6mL andVs=12.7mL . Calculate the retention factor and the partition coefficient for this solute.

Short Answer

Expert verified

a) Retention factor =k =3.59

b) Partition coefficient=k = 4.69

Step by step solution

01

Given Information:

Vr=76.2mL,Vm=16.6mL,Vs=12.7mL

02

To find the Retention factor:

Retention factor as

k=Vr-VmVm

Substitute the given data values are

k=76.2mL-16.6mL16.6mLk=3.59

03

To find the Partition coefficient;

K=k×VmVs

Substitute the given data values are

K=3.59×16.6mL12.7mLK=4.69

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Most popular questions from this chapter

(a) For the asymmetric chromatogram in Figure 23-14, calculate the asymmetry factor, BA.(b) The asymmetric chromatogram in Figure 23-14 has a retention time equal to 15.0 min and a w0.1of 44s . Find the number of theoretical plates. (c) The width of a Gaussian peak at a height equal to110 of the peak height is 4.297σ. Suppose that the peak in (b) is symmetric with A=B=22s. Use Equations 23-30 and 23-32 to find the plate number.

Butanoic acid has a partition coefficient of 3.0 (favouring benzene) when distributed between water and benzene. Find the formal concentration of butanoic acid in each phase when 100mL of 0.10M aqueous butanoic acid is extracted with 25mL of benzene (a) at pH 4.00 and (b) at pH 10.00.

Isotopic compounds (isotopologues) are separated in Figure 23-15 by repeated passage through a pair of columns. Each cycle in the figure represents

one pass through length L = 25 cm containing

L theoretical plates. The relative retention (α) is and the retention factor for

L-phenylalanine is k2=1.62. (a) The observed resolution after 10 cycles is 1.60 .

Calculate the number of theoretical plates, N , in column length L . The mixture

has passed through length 10L in 10 cycles.

(b) Find the plate height in μm.

(c) Predict the resolution expected from two cycles. The observed value is 0.71 .

A chromatographic procedure separatesof unknown mixture on a column with a length of 40cmand a diameter of 0.85cm . (a) What size column would you use to separate 100mgof the same mixture? (b) If the flow is 0.22mL/minon the small column, what volume flow rate should be used on the large column? (c) The mobile phase occupies 35%of the column volume. Calculate the linear velocity for the small column and the large column.

In chromatography, resolution is governed by (a) the number of plates, (b) relative retention, and (c) retention factor. Construct a set of graphs to show the dependence of resolution on each of these three parameters. Comment on the nature of the dependence of resolution on each of these three parameters.
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