Which of the following columns will provide:

(a) highest number of plates?

(b) greatest retention?

(c) highest relative retention?

(d) best separation?

Column1:N=1000;k2=3.9;α=1.16;resolution=0.6Column2:N=5000;k2=3.9;α=1.06;resolution=0.8Column3:N=500;k2=4.7;α=1.31;resolution=1.1Column4:N=2000;k2=2.4;α=1.24;resolution=1.5

Short Answer

Expert verified

The solution is

- a) Column 2 because it has the biggest N value (N = 5000)

- b) Column 3 because it has the largest k2value k2=4.7

- c) Column 3 because it has the largest role="math" localid="1654934451145" αvalue α=1.31

- d) Column 4 because it is the only one with baseline resolution (resolution = 1.5)

Step by step solution

01

of 3

In this task we will determine which of the columns will provide:

a) highest number of plates

b) greatest retention

c) highest relative retention

d) best separation

02

of 3

Column1:N=1000;k2=1.2;α=1.16;resolution=0.6Column2:N=5000;k2=3.9;α=1.06;resolution=0.8Column3:N=500;k2=4.7;α=1.31;resolution=1.1Column4:N=2000;k2=2.4;α=1.24;resolution=1.5

03

of 3

The solution is

- a) Column 2 because it has the biggest N value (N = 5000)

- b) Column 3 because it has the largest k2value k2=4.7

- c) Column 3 because it has the largest αvalue α=1.31

- d) Column 4 because it is the only one with baseline resolution (resolution = 1.5)

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Most popular questions from this chapter

For the separation of A and B by column 2 in Problem 23-32:

(a) If broadening is mainly due to longitudinal diffusion, how should the flow rate be changed to improve the resolution?

(b) If broadening is mainly due to the finite equilibrium time, how should the flow rate be changed to improve the resolution?

(c) If broadening is mainly due to multiple flow paths, what effect will flow rate have on the resolution?

A chromatogram with ideal Gaussian bands hastr=9minand
w1/2=2min.

(a) How many theoretical plates are present?

(b) Find the plate height if the column is 10cm long.

23-43. An infinitely sharp zone of solute is placed at the center of a column at time t=0. After diffusion for timet1 , the standard deviation of the Gaussian band is 1.0mm. After20min more, at time t2, the standard deviation is2.0mm . What will be the width after another20min , at time t3?

A layer with negligible thickness containing 10.0nmol of methano(D=1.6×10-9m2/s)was placed in a tube of water 5.00 cm in diameter and allowed to spread by diffusion. Using Equation 23-27, prepare a graph showing the Gaussian concentration profile of the methanol zone after 1.00,10.0, and 100 min. Prepare a second graph showing the same experiment with the enzyme rib nuclease(D=1.12×10-9m2/s).

(a) Write the meaning of the retention factor,k, in terms of time spent by solute in each phase. (b) Write an expression in terms of kfor the fraction of time spent by a solute molecule in the mobile phase. (c) The retention ratio in chromatography is defined as R=timeforsolventtimeforsolutetopassthroughcolumn=tmtrShow that is related to the retention factor by the equation R=1/k+1

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