Chapter 23: Q44P (page 631)
A chromatogram with ideal Gaussian bands hasand
.
(a) How many theoretical plates are present?
(b) Find the plate height if the column is 10cm long.
Short Answer
The solution is
Chapter 23: Q44P (page 631)
A chromatogram with ideal Gaussian bands hasand
.
(a) How many theoretical plates are present?
(b) Find the plate height if the column is 10cm long.
The solution is
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Get started for free23-38. What is the optimal flow rate in Figure 23-17 for best separation of solutes?
23-47. A chromatographic peak has a width,W,of and a retention volume of role="math" localid="1654842133798" . What width is expected for a band with a retention volume of ? Assume that the only band spreading occurs on the column itself.
(a) Explain why the diffusion coefficient of is greater than that of
sucrose in Table 23-1.
(b) Make an order-of-magnitude estimate of the diffusion coefficient of water
vapor in air at 298K.
(a) Why is it difficult to extract the complex of aluminum into an organic solvent but easy to extract the complex?
(b) If you need to bring the complex into the organic solvent, should you add a phase transfer agent with a hydrophobic cation or a hydrophobic anion?
A chromatographic procedure separatesof unknown mixture on a column with a length of 40cmand a diameter of 0.85cm . (a) What size column would you use to separate 100mgof the same mixture? (b) If the flow is 0.22mL/minon the small column, what volume flow rate should be used on the large column? (c) The mobile phase occupies 35%of the column volume. Calculate the linear velocity for the small column and the large column.
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