A chromatogram with ideal Gaussian bands hastr=9minand
w1/2=2min.

(a) How many theoretical plates are present?

(b) Find the plate height if the column is 10cm long.

Short Answer

Expert verified

The solution is

a)N=112b)H=0.089cm

Step by step solution

01

of 3

We have a chromatogram with perfect Gaussian bands of tr=9minand w1/2=2minin this task.

Because we have w1/2here, we used the half-width formula instead of Equation 23-30.

02

of 3

a) Here we will find the number of theoretical plates present on a column:

N=5.55tr2w1/22N=5.55×9min22min2N=112

Because we haveW1/2 here, we used the half-width formula instead of Equation 23-30

03

of 3

a)If the column is long, we will compute the plate height as follows:

H=LNH=10cm112=0.089cm

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Most popular questions from this chapter

Which of the following columns will provide:

(a) highest number of plates?

(b) greatest retention?

(c) highest relative retention?

(d) best separation?

Column1:N=1000;k2=3.9;α=1.16;resolution=0.6Column2:N=5000;k2=3.9;α=1.06;resolution=0.8Column3:N=500;k2=4.7;α=1.31;resolution=1.1Column4:N=2000;k2=2.4;α=1.24;resolution=1.5

(a) For the asymmetric chromatogram in Figure 23-14, calculate the asymmetry factor, BA.(b) The asymmetric chromatogram in Figure 23-14 has a retention time equal to 15.0 min and a w0.1of 44s . Find the number of theoretical plates. (c) The width of a Gaussian peak at a height equal to110 of the peak height is 4.297σ. Suppose that the peak in (b) is symmetric with A=B=22s. Use Equations 23-30 and 23-32 to find the plate number.

Consider a chromatography experiment in which two components with retention factors k1=4.00and k2=5.00are injected into column with N=1.00×103theoretical plates. The retention time for the less-retained component is tr1=10.0min.

(a)Calculate tmandt12.Find w12(width at half height) and w (at the base) for each peak.

(b)Using graph paper , sketch the chromatogram analogous to figure 23-7,supposing that two peaks have same amplitude(height). Draw the half widths accurately.

(c)Calculate the resolution of the two peaks and compare this value with those drawn in Figure 23-10.

Consider a chromatography column in whichVs=Vm/5 Find the retention factor if K = 3 and if K = 30 .

Why is longitudinal diffusion a more serious problem in gas chromatography than in liquid chromatography?

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