Chapter 22: QBE (page 598)
What resolving power is required to distinguishfrom?
Short Answer
The resolving power is 800 .
Chapter 22: QBE (page 598)
What resolving power is required to distinguishfrom?
The resolving power is 800 .
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Get started for free(This is a long exercise suitable for group work.) Relative intenities for the molecular ion region of several compounds are listed in arts (a)-(d) and shown in the figure. Suggest a composition for each nolecule and calculate the expected isotopic peak intensities.
a)
b)
c) m/z
d)
Find the number of rings 1 double bonds in molecules with the following compositions and draw one plausible structure for each: (a) (b)(c) fragment in a mass spectrum with the composition .
(a) To detect the drug ibuprofen by liquid chromatography– mass spectrometry, would you choose the positive or negative ion mode for the spectrometer? Would you choose acidic or neutral chromatography solvent? State your reasons.
(b)If the unfragmented ion has an intensity of 100, what should be the intensity of M+1?
Bone consists of the protein collagen and the mineral hydroxyapatite,. The content of archaeological humanskeletons measured by graphite furnace atomic absorption shedslight on customs and economic status of individuals in historicaltimes. 37Explain why La3+ is added to bone samples to suppressmatrix interference in Pbanalysis.
The molecular ion region in the mass spectrum of a large molecule, such as a protein, consists of a cluster of peaks differing by 1 Da. This pattern occurs because a molecule with many atoms has a high probability of containing one or several atoms of and . In fact, the probability of finding a molecule with only and may be so small that the nominal molecular ion is not observed. The electrospray mass spectrum of the rat protein interleukin-8 consists of a series of clusters of peaks arising from intact molecular ions with different charge. One cluster has peaks at m/z 1 961.12, 1 961.35, 1 961.63, 1 961.88, 1 962.12 (tallest peak), 1 962.36, 1 962.60, 1 962.87, 1 963.10, 1 963.34, 1 963.59, 1 963.85, and 1 964.09. These peaks correspond to isotopic ions differing by 1 Da. From the observed peak separation, fi nd the charge of the ions in this cluster. From m/z of the tallest peak, estimate the molecular mass of the protein.
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