Chapter 0: Q4-9 TY (page 1)

What mass of protein gives a corrected absorbance of $0.250$ ?

Short Answer

Expert verified

The mass of protein giving a corrected absorbance of $0.250 is 15.0 μg$ .

Step by step solution

Concept Used

Linear Calibration Curve:

The equation of the linear calibration line is:

$y (\pm s_{y}) = [m (\pm u_{m})] x + [b (= u_{b})]$

where,

$y$ is the corrected absorbance.

Corrected Absorbance = Observed Absorbance - Blank Absorbance

Calculation of Mass of Protein

The equation of the linear calibration line is:

$absorbance = m x (μg of protein) + b y = (0.00163) (μg of protein) + 0 . 004_{7}$

The numbers in the subscript denote insignificant figures.

The given corrected absorbance $(y) = 0.250$

The mass of protein is:

$\frac{= corrected absorbance - 0.0047}{0.01630}$

Mass of Protein

$= \frac{0.250 - 0.0047}{0.01630} = 15.05 = 15 . 0_{μg} (rounded off)$

Therefore, the mass of protein resulting in a corrected absorbance of $0.250 is 15.0 μg$

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What mass of protein gives a corrected absorbance of $0.250$ ?

Short Answer

Step by step solution

Concept Used

Calculation of Mass of Protein

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What mass of protein gives a corrected absorbance of 0.250?

Short Answer

Step by step solution

Concept Used

Calculation of Mass of Protein

One App. One Place for Learning.

Most popular questions from this chapter

Recommended explanations on Chemistry Textbooks

Chemical Analysis

Inorganic Chemistry

Physical Chemistry

Chemistry Branches

Making Measurements

Organic Chemistry

Study anywhere. Anytime. Across all devices.

What mass of protein gives a corrected absorbance of $0.250$ ?