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Chapter 6: Problem 1

Draw a mechanism for the topoisomerase-catalyzed cleavage of DNA that gives the free \(5^{\prime}\)-end.

Short Answer

Expert verified
The mechanism involves the enzymatic action of topoisomerase causing a break in the DNA's phosphate backbone. As a result, the strands separate, resulting in the emergence of a free 5'-end.

Step by step solution

01

Understanding Enzymatic Action of Topoisomerase

Topoisomerase is an enzyme that modulates the overwinding or underwinding of DNA. The enzyme breaks the DNA's phosphate backbone to facilitate the unwinding process.
02

Identifying the Formation of Free 5'-End

When DNA is broken by topoisomerase, it results in the release of free ends. In this case, we focus on the release of the 5'-end. This end usually has a phosphate group attached.
03

Drawing the Mechanism

For simplicity, represent DNA as a double helix structure, and topoisomerase as an entity interacting with the DNA molecule. At the cleavage site, illustrate the breaking of the DNA backbone, leading to the separation of DNA strands and the free 5'-end emerged.
04

Highlighting the Resultant 5'-End

In the final step, draw attention to the free 5'-end by marking it. This is the result of topoisomerase action.

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